| EXOSC7

Exosome component 7, also known as EXOSC7, is a gene, the protein product of which is part of the exosome complex.Predicted to enable mRNA 3'-UTR AU-rich region binding activity. Involved in RNA catabolic process and RNA processing. Part of cytosol; exosome (RNase complex); and nucleolus.EXOSC7 (Exosome Component 7) is a Protein Coding gene. Diseases associated with EXOSC7 include Pontocerebellar Hypoplasia, Type 1C and Pontocerebellar Hypoplasia, Type 1B.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.23	1.42	0.83	0.5	0.31	0.23	0.18	0.08
Corrected OD	2.15	1.34	0.75	0.42	0.23	0.15	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human EXOSC7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	83-101	96
Cell Lysates(n=8)	92-105	103

 

| Linearity

Samples were spiked with high concentrations of Human EXOSC7 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	82-95	88-96
	Average (%)	91	92
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	100